Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190. M3-M5e_manual. SpectraMax® M5 Multi-Mode Microplate Re. Opens in a new window or tab. The Molecular Devices SpectraMax M5 plate reader is dual monochromator, allowing selection of any absorbance wavelength between 200 nm and 1,000 nm, and any excitation wavelength between 250 nm and 850 nm for reading in fluorescence intensity, time-resolved fluorescence (TRF), or wavelength-selectable luminescence modes, and 400 - 750 nm. The chamber. Self Service Available. We empower scientists to advance discovery Automated, end-to-end solutions span research disciplines to help scientists discover, scale, and industrialize biology. Five preferred detection modes. Description. Serial number location on Series II and Multi-Mode plate readers. 1321N1, a human astrocytoma cell line, expresses. 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. Shop now. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader,. Thermo Scientific Microplate Reader Verification Plates Cat# 24072800. 3 or greater. In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Be sure to insert the purple (SpectraMax M5 or M5e reader) or black (FlexStation 3 reader) plate adapter in the plate carriage prior to Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Page 1 of 8 . SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. Gemini EM Reader Top and Bottom OpticsThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. Clear Filters. 12. SpectraMax M5e Multi-Mode Microplate Reader. After the plate is read, data will appear in the plate. SpectraMax ABS Plus SpectraTest ABS2 v1. Description. After the plate is read, data will appear in the plate section: 11. For users operating in a FDA 21 CFR Part 11 compliant environment, SoftMax Pro GxP Software is available, allowing user permissions, audit trails, e-signature and reporting tools. spr SpectraMax Plus 384. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. For example, microplates containing different samp les can be read using the same orThe Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. 95. The protocol contains settings for reading the plate, as well as template groups that allow a user to easily set up analysis, including calculation. For the BioVision cytotoxicity kit, 100 µL of culture medium was removed from each treated well and transferred to a solid white plate. In the M2, M5 and Plus. The guide also covers the software features, the specifications, and the accessories of the systems. 00800 665 32860. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 1. Extended linear dynamic range. UV/Vis Photometric Performance: Wavelength Range. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. 00. 40266The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Features. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. 0 out of 5 $ 5,999. Reader Documentation Version No. #655801. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 0200-7027. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. B. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Figure 1. Abs). SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. 0 . SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Add to Cart. ”The SpectraMax 190 reader can be integrated with Molecular . For SpectraMax L or LMax II, LMax II. 95. spr SpectraMax iD5 SpectraTest ABS2 v1. . SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact u@osu. e. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Detection cartridge modularity allows for easy addition of new read modes within minutes without. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. es •. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. D 3 1. Here the delta mP was 336, and Z’ factor was 0. 1. Molecular devices SpectraMax M5 Pdf User Manuals. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Choose from a three- (M3), four- (M4), orThey include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate. Products : SpectraMax M2/M2e reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, Gemini EM reader, SpectraMax Plus 384 reader. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. The SpectraMax ® i3x. SpectraMax i3x reader settings were configured as indicated in. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. Perkin Elmer 1420-2560 Victor X Series Plate Reader Sipper. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Choose from a three- (M3), four-. 1. I accidentally left a plate in my lab's M5 SpectraMax plate reader overnight. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Sunnyvale, California -- October 5, 2017. book Page 1 Wednesday, October 12, 2011 4:56 PMThe SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. The SpectraMax M2 and M2e are versatile and reliable. The M5 can read volumes down to 2uL with the. 306 plate air cal fail,low light. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. 18 x 14 x 8 ins. Here the delta mP was 336, and Z’ factor was 0. 0200-0729. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. HTRF-compatible SpectraMax Multi-Mode Readers. 12. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. SpectraMax M5 Multi-Mode Microplate Reader. There is currently no charge for using this plate reader. We show how the DuoLuc reporter assay and SpectraMax iD5 reader are used to detect nuclear factor-κB (NF-κB) activation in a mammalian cell model. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. SpectraMax L reader, SpectraMax M5/M5e reader What is the StakMax timing with SpectraMax Plate Readers? If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the StakMax are as follows:. The dual monochromator optics allow the. Figure 2-23: Bottle holder To install the bottle holder: 1. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraTest ABS2 Absorbance Validation Package performance tests. 2. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. SpectraMax M5 Plate Reader SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 305 reference level saturation. Abs). Please talk to your your local Tecan representative for the right configuration which suits your application demands. Supported Plates and Cuvettes. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. • Tel. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. plate type, read area and number of images per well, plus specify positive/negative wells. Microplate Reader Shelf. These robust readers have been placed in labs from. 2 Software (multiple versions available) Windows 7 PC 60 day warranty All necessary communication electrical cables The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Microplate readers’ standard detection modes. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. easy to use and read multiple plates very quickly. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. 25 pM (0. In an analogous manner, the same sample names were assigned to a CuvetteSet section. Click the OK button. When a feature or capability applies to only certain readers, this exception is noted. series measured in a 96-well plate in the SpectraMax M5 and the Analyst GT. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A 5 1. edu, 814-863-3503. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. SpectraMax M5 reader SpectraMax M5e. Trends in Immunology. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. 2. flexibility of monochromator-based systems. In this. edu, 814-863-3503. M5 and M5e Multi-Mode Microplate Readers User Guide SpectraMax M3, M4, M5. Please contact Tatiana to reserve the plate reader time: tnl1@psu. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Description. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 12. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Select Update Driver. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 1. 0. Description 1. Find Molecular Devices SpectraMax M3/M4/M5/M5e Only Molecular Devices microplate plate readers offer the In need of a service manual for the SpectraMax M5 Find Molecular Devices SpectraMax Plus 384 – Forums, 190 – 1000 nm, tunable Molecular Devices SpectraMax Plus 384 Plate Reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. If the computer is connected to the Internet: Select Search Automatically for updated driver software. However, when a specific probe is not available, or it causes off-target effects on the. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. See our app note for details:. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. A microplate reader asThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. M5 E -HTRF Readers. 6: bioNova científica, s. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. SpectraMax M5 Multi-Mode Microplate Reader. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. youusehighprofile(6-well,12-well,24-well,or48-well)plates,youmustremovethe plateadapterfromtheplatedrawer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. edu, 814-863-3503. Choose from a three- (M3), four- (M4), or Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Plate pre-read measurements were made in a Costar UV plate. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Page 1 of 8 . The chamber. Microplate Readers include the M3, M4,. Bioz Stars score: 86/100, based on 1 PubMed citations. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. Features. Then the plate was returned to the. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates FL1, Gemini EM reader, Gemini XPS reader, SpectraMax M5/M5e reader My SpectraTest FL one plate is failing for LLD for the wet test. Standard curves were plotted using SoftMax® Pro Software (Figure 1). A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. 000 OD. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. SpectraTest ABS2 Absorbance Validation Package performance tests. 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. readers, the vertical light beam results in a pathlength that depends on the vol-. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Plate readers can vary in attributes such as microwell plate format, throughput, optical system, wavelength range, and more. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Ensure data accuracy of your reader. 0 out of 5 $ 16,499. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. Bottom-reading is usually recommended for cell-based assay with adherent cells. Choose from a three- (M3), four-. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. Comes from Lab closure Approx. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Information on how to reserve the plate readerThe GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. Typical LLDs were approximately 1–2 fg/well in the Analyst GT and 2–3 fg/well in the SpectraMax M5. Bookings are. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). The SpectraMax M3 Microplate Reader is supplied with SoftMax Pro Data Acquisition and Analysis Software, Molecular Devices' industry leading all-in-one software. The SpectraMax M5 e reader has the same performance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF. For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. We would like to show you a description here but the site won’t allow us. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Fluorescence Microplate Reader Comparison Chart. In the M2, M5 and Plus 384, the light. 3: The cuvette chamber. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. After the plate is read, data will appear in the plate section: 11. 100 µL of AK Reagent Working Solution was added to each well, and the plate was read within 30 minutes. 04 to 3. 40266 Email: drugdiscoverytech@lifetech. Published Dec 16, 2020 | Updated Dec 16, 2020. Gemini EM. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. Plate reader instructions Computer. Instructions. Read More. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Developed with Promega reagents to provide a. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. edu The content of this site is published by the site owner(s) and is not a statement of advice, opinion, or information pertaining to The Ohio State University. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Category 2 and 3 microplate readers use gradient heating as well. Weighs about - 23 lbs. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. It was then read from the top and bottom on the SpectraMax iD3 reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. 2 with 1000 cells. 0 OD for earlier models. 40266 Email: drugdiscoverytech@lifetech. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 03 in x 3. SpectraMax Injector Cartridge with SmartInject™ Technology. The M5 can read volumes down to 2uL with the. The following document is intended. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. A solution for low volume applications. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Detection modalities. 2. l. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. e. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). It features a modular design that allows users to add or change detection cartridges according to their needs. Reader Documentation Version No. 7°C and the bottom to 36. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. For more information please visit us at: M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. D 1 1. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). book Page 1 Wednesday, July 7, 2010 2:09 PMThe Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Test up to 3. SpectraMax M5e Multi-Mode Microplate Reader. SpectraMax® M5 Microplate Reader Molecular Devices LLC Applications: Absorbance,. com 10. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. These robust readers have been placed in labs from Antarctica to. 7. Molecular Devices. No additional manuals are available. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. View the Molecular Devices website. When the user applies a set temperature of 37°C, the top is heated to 37. Pathcheck Pathlength Measurement Technology. Description 1. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 1) Select the correct plate and the wells that you want to measure. For wavelengths below 220 nm, only quartz plates are to be used. Figure 10: The Plate section Template Editor, showing the series set up. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integratedWhat types of plates can be used with StakMax? SBS Standard 96 and 384 well plates with plate height of 12-16 mm are recommended. HTRF is a. 313 reference gain check fail. The dynamic range of the assay was approximately 5 orders of magnitude. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. We would like to show you a description here but the site won’t allow us. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Read More. Our 15 years of experience means we understand these instruments as well as the manufacturer, and it allows us to provide top-quality service. Molecular devices SpectraMax M5e Pdf User Manuals. Also refer to this Multimode Reader Comparison Chart. Add to Cart. Robust, high-value microplate readers that can run samples based on pre-defined protocols and. Dynamic Range. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. FL1 Validation Plate automatically validates the performance of the reader. FlexStation II 384. PURPOSE. Put the plate in the SPECTRAmax PLUS drawer, then click the Read but-ton in the Plate section’s Status Bar. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Access this by going to Help >. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. 6 for further experiment, so I'm confused which i would choose for OD value determination. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Recertification is recommended every two years. Spectramax M5. Molecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. 383 inches whereas the maximum allowed is 5. columns 1, 3, 5, 7, 9 and 11 were seeded with 200,000 mock-transfected cells per well. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. For prep samples treated with the ELISA kit reagents, it is fast and user friendly. Copia stocks a full line of used microplate readers. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Molecular Devices microplate readers. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. 2. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. TR-FRET assay. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Call 516-939-0888 to check. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never. 9000-0756. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. 005) Photometric PrecisionThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. SpectraMax i3x Multi-Mode Microplate Re. SpectraMax® 340PC384 Microplate Reader for acquiring data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments. SpectraMax M2. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orSPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. Experimental details and customization of the group table. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence.